DIAGNOSITIC KITS & LAB REQUIREMENTS

List Of Essential Molecular Diagnostic Equipments

Coronaviruses are single-stranded RNA viruses that belong to the Coronaviridae family. It is the emergence of the most current coronaviruses, SARS-CoV-2 (the virus responsible for the disease Covid-19), which has the research community’s attention. The high infection and mortality rates has made the development of testing kits and a vaccine paramount.

 

COVID-19 consists of four major proteins including a protruding glycoprotein (spike), membrane, envelope, and nucleocapsid. Given its multi-protein structure scientists are utilizing EIA immunoassays, ELISA immunoassays, reverse transcription PC assays (RT-PCR) and real-time quantitative PCR assays (qRT-PCR). 

The Centers for Disease Control and Prevention (CDC) is providing test kits for public health laboratories (PHLs) in order to perform real-time RT-polymerase chain reaction (rRT-PCR) detection for SARS-CoV-2. [1] Full protocol for CDC 2019-Novel Coronavirus (2019-nCoV) is available for download and intended for the presumptive qualitative detection of nucleic acid from the 2019-nCoV. [2]

 

Coronavirus assays and various staple lab equipment products are utilized in coronavirus testing which includes, but is not limited to:

  • RT-PCR Reagents
  • Real-Time PCR Detection Systems
  • Nucleic Acid Extraction Systems
  • PCR Work Stations
OTHER EQUIPEMENT: 
  1. Biosafety cabinet level two
  2. Biosafety cabinet (hood)
  3. Deep freezer
  4. Refrigerator  temprature 2-8
  5. Hot plate 0.2 ml 96 well plate
  6. Dry hot oven sterillizer
  7. Autoclave
  8. Vortex
  9. Micro centrifuge(conical 1.5 ml tube)
  10. Microcentrifduge tube fack
  11. Chemistry analyzer 200 test per hour and above
  12. Hematology analyzer
  13. Electrolyte analyzer
  14. Coagulation analyzer
 For more information write to Shashi Kumar ( shashi@mozocare.com) or call +91.8826883200

References:

[1] Information for Laboratories: 2019-NCoV | CDC. 9 Feb. 2020, https://www.cdc.gov/coronavirus/2019-ncov/lab/index.html.

[2] CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel. https://www.fda.gov/media/134922/downloa

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